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An amniocentesis is performed on a woman whose last two pregnancies have resulted in stillbirths due to hemolytic disease of the newborn.A screening test performed at the hospital is positive for bilirubin,and the specimen is sent to a reference laboratory for a bilirubin scan.Doctors are concerned when the report comes back negative,and they question if the:


A) Correct specimen was sent
B) Specimen was refrigerated
C) Specimen was exposed to light
D) Specimen reached the reference laboratory within 30 minutes

E) A) and B)
F) A) and C)

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The foam,or shake,test is a screening test for amniotic fluid:


A) Bilirubin
B) Surfactants
C) Alpha fetoprotein
D) Phosphatidyl glycerol

E) None of the above
F) All of the above

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Most laboratories detect the presence of phosphatidylglycerol using thin-layer chromatography.

A) True
B) False

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Meconium is composed of bowel constituents.

A) True
B) False

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Decreased levels of phosphatidyl glycerol in amniotic fluid can be associated with:


A) Hemolytic disease of the newborn
B) Toxemia of pregnancy
C) Hyaline membrane disorder SS
D) Intrauterine death

E) B) and C)
F) A) and B)

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Hemolytic disease of the newborn is caused by:


A) Abnormal maternal hemoglobins
B) Abnormal fetal hemoglobins
C) Maternal antibodies
D) Fetal antibodies

E) None of the above
F) B) and C)

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A second amniocentesis is performed on a diabetic woman believed to be at approximately 35 weeks gestation.She is pregnant with her second child,and the bilirubin A450 on the first amniocentesis was in zone 2 of the Liley graph. -Based on the information provided,if the bilirubin A450 is now in zone 3,what additional testing should be performed?


A) Alpha fetoprotein
B) Lamellar bodies
C) Maternal antibody screen
D) Fern test

E) B) and C)
F) A) and B)

Correct Answer

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Interference with the amniotic fluid bilirubin analysis on centrifuged fluid is most likely caused by:


A) Fetal cells
B) Oxyhemoglobin
C) Albumin
D) Creatinine

E) C) and D)
F) None of the above

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The test for amniotic fluid lamellar bodies uses which of following?


A) Fluorescence
B) Polarizing microscopy
C) A spectrophotometer
D) An automated cell counter

E) All of the above
F) C) and D)

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An amniocentesis is performed on a woman believed to be at approximately 15 weeks gestation.A possible abnormality has been observed on her sonogram,and her serum alpha fetoprotein is elevated. -Using the information provided,what type of fetal distress is suspected?


A) Hemolytic disease of the newborn
B) Polyhydramnios
C) Oligohydramnios
D) Neural tube disorder

E) A) and D)
F) B) and C)

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Production of both lecithin and sphingomyelin increases after 35 weeks gestation.

A) True
B) False

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A second amniocentesis is performed on a diabetic woman believed to be at approximately 35 weeks gestation.She is pregnant with her second child,and the bilirubin A450 on the first amniocentesis was in zone 2 of the Liley graph. -Based on the information provided,should an L/S ratio or an Amniostat test be performed?


A) L/S ratio
B) Amniostat

C) A) and B)
D) undefined

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The principle of the shake test and foam stability index is that phospholipids:


A) Reduce the surface tension of an alcoholic solution
B) Increase the surface tension of an alcoholic solution
C) Release carbon dioxide in an alcoholic solution
D) Release carbon dioxide when agitated

E) A) and B)
F) A) and C)

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In the determination of fetal lung maturity,which of the following components is produced by the body at the 35th week of gestation?


A) Lecithin
B) Phosphatidyl glycerol
C) Lamellar body
D) Acetylcholinesterase

E) B) and C)
F) A) and D)

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The Amniostat agglutination test is performed on amniotic fluid from pregnant women to detect the presence of:


A) Lecithin
B) Phosphatidyl glycerol
C) Sphingomyelin
D) Creatinine

E) A) and D)
F) B) and C)

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The volume of amniotic fluid increases after the first trimester as a result of:


A) Increased maternal blood flow to the fetus
B) Diffusion of interstitial fluid
C) Production of fetal urine
D) Increased fetal swallowing

E) None of the above
F) All of the above

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An amniocentesis is performed on a woman believed to be at approximately 15 weeks gestation.A possible abnormality has been observed on her sonogram,and her serum alpha fetoprotein is elevated. -Using the information provided,what is the normal value of amniotic fluid alpha fetoprotein?


A) 0.5 MoM
B) 1.0 MoM
C) 1.5 MoM
D) 2.0 MoM

E) B) and C)
F) A) and C)

Correct Answer

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The method of choice for testing amniotic fluid contaminated with hemoglobin for fetal lung maturity is:


A) Bilirubin levels
B) Amniostat
C) Thin-layer chromatography
D) Foam stability

E) None of the above
F) B) and D)

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An amniotic fluid optical density at 650 nm of 0.180 correlates with:


A) A lamellar body count over 32,000/L
B) A lack of phosphatidylglycerol
C) Hemoglobin contamination in the specimen
D) An L/S ratio of 1.5

E) None of the above
F) All of the above

Correct Answer

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When an L/S ratio by thin-layer chromatography is performed,a mature fetal lung will show:


A) Sphingomyelin twice as concentrated as lecithin
B) No sphingomyelin
C) Lecithin twice as concentrated as sphingomyelin
D) Equal concentrations of lecithin and sphingomyelin

E) A) and C)
F) C) and D)

Correct Answer

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