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Thymine dimers are dealt with by


A) no repair mechanisms.
B) photoreactivation repair.
C) SOS repair.
D) excision repair.
E) photoreactivation repair AND excision repair.

F) A) and C)
G) B) and E)

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The formation of a covalent bond between two adjacent thymines is caused by


A) mustard gas.
B) alkylating agents.
C) microwave radiation.
D) UV radiation.

E) A) and B)
F) A) and C)

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Planar molecules used as chemical mutagens are called


A) nitrous oxide.
B) base analogs.
C) alkylating agents.
D) intercalating agents.

E) None of the above
F) B) and C)

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Which is not true about a crown gall tumor?


A) It is a bacterial infection of plants.
B) It requires a plasmid.
C) It produces a large amount of opines that neither the plant nor bacteria synthesizes.
D) It is due to the incorporation of bacterial plasmid DNA into the plant chromosome.
E) All of the choices are true.

F) A) and E)
G) A) and B)

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Direct selection involves inoculating cells onto growth media on which


A) the mutant but not the parental cell type will grow.
B) the mutation will be reversed.
C) the nutrients necessary for mutation to occur are present.
D) the mutagen is present.

E) B) and C)
F) A) and B)

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Plasmids often carry the information for antibiotic resistance.

A) True
B) False

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Transposons may leave a cell by incorporating themselves into a plasmid.

A) True
B) False

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Double-stranded DNA enters the recipient cell during transformation.

A) True
B) False

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DNA repair mechanisms occur


A) only in prokaryotes.
B) only in eukaryotes.
C) in both eukaryotes and prokaryotes.
D) in neither eukaryotes or prokaryotes.

E) A) and B)
F) A) and C)

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F plasmids and oftentimes R plasmids are both able to code for production of a pilus.

A) True
B) False

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A clever technique that streamlines the identification of auxotrophic mutants is


A) gas chromatography.
B) replica plating.
C) direct selection.
D) reversion.

E) All of the above
F) None of the above

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To maximize the number of thymine dimer mutations following UV exposure,should you keep human cells in tissue culture in the dark,in the light,or does it matter at all?


A) The dark-light will activate the photorepair systems that can break thymine dimers induced by UV light.
B) The light-it's important to keep on producing the thymine dimers by keeping the plate exposed to light as much as possible.
C) It's best to alternate light and dark every hour to increase the chances that thymine dimers will form in the human cells,but still keep the photorepair systems from correcting them as they are formed.
D) It doesn't matter-human cells don't possess the enzymes needed for photorepair of thymine dimers.

E) A) and B)
F) A) and C)

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The Ames test determines antibiotic sensitivity of a bacterium.

A) True
B) False

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Transposons


A) are informally known as jumping genes.
B) may cause insertion mutations.
C) may cause knockout mutations.
D) were first recognized in plants.
E) All of the choices are correct.

F) A) and B)
G) A) and E)

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Antibiotics


A) cause mutations to occur.
B) may act as alkylating mutagens.
C) provide an environment in which preexisting mutants survive.
D) increase the rate of spontaneous mutation.

E) B) and C)
F) C) and D)

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DNA polymerase is able to proofread the DNA sequence.

A) True
B) False

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Competent cells


A) are able to take up naked DNA.
B) are antibiotic resistant.
C) occur naturally.
D) can be created in the laboratory.
E) are able to take up naked DNA,occur naturally,AND can be created in the laboratory.

F) D) and E)
G) A) and C)

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Irradiation of cells with ultraviolet light may cause


A) four nucleotides to covalently bind together.
B) thymine dimers.
C) adenine complementary base pairing with cytosine.
D) the addition of uracil.

E) B) and D)
F) B) and C)

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The study of the crown gall tumor found


A) a bacterial plasmid promoter that was similar to plant promoters.
B) an R plasmid.
C) incorporation of the bacterial chromosome into the plant.
D) incorporation of the plant chromosome into the bacteria.

E) C) and D)
F) All of the above

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