Question 1

Regions of a specimen with higher electron density scatter ___________ electrons and,therefore,appear __________ in the image projected onto the screen of a transmission electron microscope.

Accepted Answer

A)  more; lighter
B)  more; darker
C)  fewer; darker
D)  fewer; lighterE) A) and D)
F) A) and C)

Question 2

Prior to staining,smears of microorganisms are heat-fixed in order to

Accepted Answer

A)  allow eventual visualization of internal structures.
B)  ensure removal of dust particles from the slide surface.
C)  attach it firmly to the slide.
D)  create small pores in cells that facilitates binding of stain to cell structures.E) A) and B)
F) B) and D)

Question 3

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the distance between the...

Question 4

The Gram-staining procedure is an example of ________________________.

Accepted Answer

A)  simple staining
B)  negative staining
C)  differential staining
D)  fluorescent stainingE) All of the above
F) C) and D) C

Question 5

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.

Accepted Answer

A)  phase-contrast
B)  electron
C)  fluorescence
D)  densitometricE) None of the above
F) C) and D) A

Question 6

The instrument that produces a bright image of the specimen against a dark background is called a (n) __________ microscope.

Accepted Answer

A)  phase-contrast
B)  electron
C)  bright-field
D)  dark-fieldE) A) and B)
F) B) and C)

Question 7

Mordants increase the binding between a stain and specimen.

Accepted Answer

A) True 
 B)False

Question 8

The _________________ microscope is capable of atomic resolution of specimens,even when they are immersed in water.

Accepted Answer

The answer of The _________________ microscope is capable of atomic...

Question 9

You are researching the structure of a transmembrane protein.Which type of microscopy would provide you the best view of this protein?

Accepted Answer

A)  Bright field microscopy
B)  Scanning electron microscopy
C)  Transmission electron microscopy
D)  Atomic force microscopyE) C) and D)
F) A) and D)

Question 10

Thin films of bacteria that have been air-dried onto a glass microscope slide are called __________.

Accepted Answer

The answer of Thin films of bacteria that have been...

Question 11

It is possible to build a light microscope capable of 10,000* magnification,but the image would not be sharp because resolution is independent of magnification.

Accepted Answer

A) True 
 B)False

Question 12

It was possible to view viruses only after the invention of the electron microscope because they are too small to be seen with a light microscope.

Accepted Answer

A) True 
 B)False

Question 13

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Accepted Answer

A)  optical density
B)  refractive index
C)  optical density and refractive index
D)  neither optical density nor refractive indexE) All of the above
F) B) and D)

Question 14

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

Accepted Answer

A) True 
 B)False

Question 15

Negative staining with India ink can be used to reveal the presence of capsules that surround bacterial cells.

Accepted Answer

A) True 
 B)False

Question 16

In the Gram-staining procedure,the counterstain is

Accepted Answer

A)  iodine.
B)  safranin.
C)  crystal violet.
D)  alcohol.E) None of the above
F) A) and C)

Question 17

The __________ is the point at which a lens focuses parallel beams of light.

Accepted Answer

The answer of The __________ is the point at which...

Question 18

A 30* objective and a 20* ocular produce a total magnification of

Accepted Answer

A)  230*.
B)  320*.
C)  50*.
D)  600*.E) All of the above
F) None of the above D

Question 19

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Accepted Answer

A) True 
 B)False

Question 20

Confocal microscopes,in combination with specialized computer software,can be used to create three-dimensional images of cell structures.

Accepted Answer

A) True 
 B)False

Exam 2: Microscopy

Regions of a specimen with higher electron density scatter ___________ electrons and,therefore,appear __________ in the image projected onto the screen of a transmission electron microscope.

Correct AnswerverifiedShow Answer

Prior to staining,smears of microorganisms are heat-fixed in order to

Correct AnswerverifiedShow Answer

The __________ is the distance between the center of a lens and the point at which it focuses parallel beams of light.

Correct AnswerverifiedShow Answer

The Gram-staining procedure is an example of ________________________.

Correct AnswerverifiedC

An instrument that magnifies slight differences in the refractive index of cell structures is called a (n) __________ microscope.

Correct AnswerverifiedA

The instrument that produces a bright image of the specimen against a dark background is called a (n) __________ microscope.

Correct AnswerverifiedShow Answer

Mordants increase the binding between a stain and specimen.

Correct AnswerverifiedShow Answer

The _________________ microscope is capable of atomic resolution of specimens,even when they are immersed in water.

Correct AnswerverifiedShow Answer

You are researching the structure of a transmembrane protein.Which type of microscopy would provide you the best view of this protein?

Correct AnswerverifiedShow Answer

Thin films of bacteria that have been air-dried onto a glass microscope slide are called __________.

Correct AnswerverifiedShow Answer

It is possible to build a light microscope capable of 10,000* magnification,but the image would not be sharp because resolution is independent of magnification.

Correct AnswerverifiedShow Answer

It was possible to view viruses only after the invention of the electron microscope because they are too small to be seen with a light microscope.

Correct AnswerverifiedShow Answer

Immersion oil can be used to increase the resolution achieved with some microscope lenses because it increases the __________ between the specimen and the objective lens.

Correct AnswerverifiedShow Answer

A light microscope with an objective lens numerical aperture of 0.65 is capable of allowing two objects 400 nm apart to be distinguished when using light with a wavelength of 420 nm.

Correct AnswerverifiedShow Answer

Negative staining with India ink can be used to reveal the presence of capsules that surround bacterial cells.

Correct AnswerverifiedShow Answer

In the Gram-staining procedure,the counterstain is

Correct AnswerverifiedShow Answer

The __________ is the point at which a lens focuses parallel beams of light.

Correct AnswerverifiedShow Answer

A 30* objective and a 20* ocular produce a total magnification of

Correct AnswerverifiedD

In order to stain flagella so that they may be readily observed by light microscopy,it is usually necessary to increase their thickness.

Correct AnswerverifiedShow Answer

Confocal microscopes,in combination with specialized computer software,can be used to create three-dimensional images of cell structures.

Correct AnswerverifiedShow Answer

Regions of a specimen with higher electron density scatter _ electrons and,therefore,appear in the image projected onto the screen of a transmission electron microscope.

Correct Answer
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