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The use of lysozyme during the preparation of cheeses and wines is effective in reducing the numbers of


A) fungi.
B) viruses.
C) bacteria.
D) prions.
E) all microbes.

F) A) and E)
G) A) and D)

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Refrigeration is an effective germicidal method.

A) True
B) False

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Formalin (37% formaldehyde) is used for


A) antisepsis.
B) disinfection.
C) sterilization.
D) sanitization.
E) both disinfection and sterilization.

F) B) and C)
G) B) and E)

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Ultra-high-temperature sterilization effectively


A) reduces microbes that cause spoilage.
B) removes only mesophilic microbes.
C) reduces microbes that cause disease.
D) removes all microbes that cause diseases or spoilage.
E) reduces microbes that cause disease or spoilage.

F) B) and D)
G) D) and E)

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The deadliest, most contagious microbes are studied under conditions of (BSL-4/BSL-3/BSL-2/BSL-1) containment.

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Which of the following is used for microbial control in fresh fruits and vegetables?


A) X rays
B) ultraviolet light
C) electron beams
D) microwaves
E) gamma rays

F) A) and B)
G) A) and C)

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Boiling water for 10 minutes is effective in ridding it of


A) actively growing bacteria.
B) enveloped viruses.
C) bacterial endospores.
D) protozoan cysts.
E) both growing bacteria and enveloped viruses.

F) C) and D)
G) A) and C)

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Elements such as iodine, chlorine, and bromine are examples of (oxidizers/halogens/metals), which are the basis for many effective antimicrobial agents.

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The physical removal of microbes is called (antisepsis/degerming/disinfection).

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Methods of microbial control called ________ arrest the growth of microbes.


A) microbicial
B) microbistatic
C) sanitizing
D) degerming
E) antiseptic

F) B) and D)
G) B) and C)

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Which of the following is NOT a feature associated with filtration?


A) nitrocellulose or plastic membrane filters
B) sterilization of heat-sensitive materials
C) varying thicknesses of membrane filters used
D) use of HEPA filters to filter air
E) ability of some filters to trap viruses and proteins

F) A) and E)
G) A) and B)

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Protozoan cysts are resistant to many disinfection methods.

A) True
B) False

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Heavy metal and oxidizing agent disinfectants damage (DNA/enzymes/membranes), interfering with microbial metabolism.

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Antimicrobial agents that damage nucleic acids also affect


A) the cell wall.
B) the cell membrane.
C) the viral envelope.
D) endospores.
E) protein synthesis.

F) None of the above
G) D) and E)

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Washing dishes in the dishwasher with detergent and hot water is a ________ process.


A) antiseptic
B) disinfecting
C) sterilizing
D) sanitizing
E) degerming

F) D) and E)
G) C) and D)

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Which of the following is the most appropriate pairing of microbe and biosafety level?


A) E. coli, BSL-3
B) anthrax, BSL-1
C) Ebola, BSL-2
D) methicillin-resistant Staphylococcus aureus (MRSA) , BSL-2
E) tuberculosis, BSL-1

F) A) and B)
G) B) and C)

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D

Quaternary ammonium compounds are disinfectants effective against


A) endospores.
B) mycobacteria.
C) nonenveloped viruses.
D) Pseudomonas aeruginosa.
E) enveloped viruses.

F) C) and D)
G) C) and E)

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Which of the following describes flash pasteurization?


A) heating at 63°C for 30 minutes
B) heating at 72°C for 15 seconds
C) heating at 72°C for 15 minutes
D) heating at 134°C for one second
E) passing liquid through steam at 140°C

F) B) and D)
G) A) and B)

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A student is shopping for antibacterial hand cleansers and is trying to decide which one to buy. One is a "waterless" hand gel containing 70% isopropanol, the second is an "antibacterial" hand soap containing triclosan (a phenolic), and the third is a wipe that lists benzethonium chloride (a synthetic quaternary ammonium salt) as the active ingredient. Compare and contrast these cleansers in terms of the action of the antimicrobial ingredient and the level of disinfection (degerming, germistatic, germicidal).

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All three are germicidal, although not all to the same degree.The alcohol of the waterless hand cleaner is a germicide that disrupts cytoplasmic membranes and denatures proteins.It is not effective against bacterial endospores or fungal spores, and it has limited effect on nonenveloped viruses.The alcohol evaporates quickly, so the germicidal effect is short term.The waterless hand cleaner is an intermediate-level disinfectant and is not an effective degermer.The hand soap contains a phenolic, which also damages cytoplasmic membranes and denatures proteins, and is effective on the same range of microbes as the alcohol.Phenolics are intermediate-level disinfectants that persist on surfaces for long periods of time, providing extended disinfection.If used with running water and the hands are vigorously rubbed, the hand soap can be an effective degermer.Synthetic "quats" (quaternary ammonium compounds) disrupt cell membranes.They are effective against fungi, enveloped viruses, and most bacteria, but not against nonenveloped viruses, nor on endospores.Quats are low-level disinfectants that are germicidal for some microbes.The action of using and discarding the wipe provides some degerming effect.

The process of filtration is a(n)


A) disinfectant method.
B) sterilizing method.
C) sanitization method.
D) antiseptic procedure.
E) ineffective method for removing microbes.

F) All of the above
G) B) and C)

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B

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